Chondrocyte culture method for promoting chondrocyte proliferation and maintaining chondrocyte phenotype and application

The invention relates to a chondrocyte culture method capable of promoting chondrocyte proliferation and maintaining chondrocyte phenotypes. The method is realized by introducing modified citrus pectin (MCP) into a cartilage cell culture system, MCP is independently added into a cartilage cell culture solution, and the cartilage cells can also be planted in a biological material or a scaffold material of the composite MCP, so that the effect of the MCP on the cartilage cells is effectively utilized, and the cartilage cells can be cultured. Proliferation of cartilage cells is promoted, the phenotype of the cartilage cells is stably maintained, and the method can be used for construction of tissue engineered cartilage based on MCP and regeneration and repair of cartilage tissue defects. 本发明涉及一种具有促进软骨细胞增殖并维持软骨细胞表型的软骨细胞培养方法。该方法是通过在软骨细胞培养体系中引入改性柑橘果胶（MCP）实现，通过单独添加MCP至软骨细胞培养液中，也可以通过将软骨细胞种植于复合MCP的生物材料或支架材料，从而有效利用MCP对软骨细胞的作用，促进软骨细胞的增殖并稳定维持软骨细胞表型，该方法可用于基于MCP的组织工程化软骨构建和软骨组织缺损的再生修复。