Collagen porous microspheres as well as preparation method and application thereof

The invention discloses a collagen porous microsphere as well as a preparation method and application thereof. The preparation method of the collagen porous microspheres comprises the following steps: mixing collagen and an acidic solution, dropwise adding the mixture into a glutaraldehyde aqueous solution through electrostatic spinning, and carrying out crosslinking, wherein the mass percent of the type I collagen in the collagen is 90% or above; the collagen accounts for 0.1%-2% of the acidic solution in percentage by mass; the voltage of the electrostatic spinning is 10-20 kV; cross-linking comprises chemical cross-linking and radiation cross-linking; the mass percent of glutaraldehyde in the glutaraldehyde aqueous solution is 0.08%-0.7%. The collagen porous microspheres prepared by the invention have mutually communicated outer and inner pores, extremely low mass density and huge specific surface area, ensure excellent biological activity and mechanical properties of collagen, provide a good regeneration