Tissue culture method of veratrum nigrum

The invention provides a veratrum nigrum tissue culture method, and belongs to the technical field of medicinal plant tissue culture. The veratrum nigrum tissue culture method comprises the steps of selection, disinfection and inoculated culture of an explant, wherein the explant is a veratrum nigrum bulb; disinfection comprises disinfection for 10-15 minutes by using a Tween 80 solution with the volume concentration of 0.3%-0.5%, disinfection for 2-5 minutes by using an alcoholic solution with the volume concentration of 95%, disinfection for 2-5 minutes by using a NaClO solution with the effective chlorine concentration of 2%-5%, and disinfection for 10-15 minutes by using a NaClO solution with the effective chlorine concentration of 0.2%-0.5%. According to the method, the veratrum nigrum bulbs are used as the explants for tissue culture, seasonal limitation is avoided, and in cooperation with a proper explant disinfection method, the required time for obtaining the sterile veratrum nigrum seedlings is shor