Recombinant escherichia coli as well as preparation method, cryopreservation method and application thereof

The invention relates to the field of biological detection of toxicity of oil field chemicals, and discloses recombinant escherichia coli Escherichia coli DH5 alpha pGEN-luxCDABE as well as a preparation method, a cryopreservation method and application of the recombinant escherichia coli coliDH5 alpha pGEN-luxCDABE. The preparation method of the recombinant Escherichia coli comprises the following steps: stably transferring a pGENluxCDABE low-copy plasmid carrying luxCDABE into Escherichia coli DH5 alpha (Escherichia coli DH5 alpha); the recombinant Escherichia coli DH5 alpha pGEN-luxCDABE disclosed by the invention can be used for detecting the biotoxicity of the oilfield chemical agent, and the cryopreservation method disclosed by the invention can be used for prolonging the preservation period of a strain, improving the survival rate of the strain, ensuring the luminous intensity of the strain and improving the use instantaneity and the transportation convenience of the recombinant luminous strain. 本发明涉及油