Application of NbPSK3 gene in tobacco turnip mosaic virus resistance

The invention discloses application of an NbPSK3 gene in tobacco turnip mosaic virus resistance, and relates to the technical field of gene engineering, and the NbPSK3 gene sequence is shown as SEQ ID NO: 1. NbPSK3 gene sequence information is utilized to amplify the gene, an overexpression vector a...

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Hauptverfasser: WANG CHAONAN, LI PENGBAI, LI HONGLIAN, ZHANG CHAO, JING XINXIN, SUN BINGJIAN, WANG PENGYUE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses application of an NbPSK3 gene in tobacco turnip mosaic virus resistance, and relates to the technical field of gene engineering, and the NbPSK3 gene sequence is shown as SEQ ID NO: 1. NbPSK3 gene sequence information is utilized to amplify the gene, an overexpression vector and a silence vector are constructed, it is found that the overexpression NbPSK3 gene can significantly enhance the resistance of nicotiana benthamiana to TuMV, the silence NbPSK3 gene is beneficial to TuMV infection, and it is indicated that the NbPSK3 gene is highly related to the resistance of the tobacco turnip mosaic virus. According to the invention, an application foundation is laid for research on a molecular mechanism of turnip mosaic virus resistance and cultivation of a new variety of turnip mosaic virus resistant tobacco, and meanwhile, new reference is provided for screening of new antiviral genes and cultivation of resistant plants. 本发明公开了NbPSK3基因在烟草抗芜菁花叶病毒中的应用,涉及基因工程技术领域,所述NbPSK3基因序列如SEQ ID NO:1所示。本发明