Method for improving tolerance of trichoderma harzianum to DMI fungicides by using resistance mutant gene

The invention discloses a method for improving the tolerance of trichoderma harzianum to DMI fungicides by using a resistance mutant gene, belongs to the technical field of biology, and particularly relates to a method for obtaining a trichoderma harzianum DMI drug-resistant strain by heterologous expression of the optimized resistance mutant gene of sphaerotheca graminearum in a trichoderma harzianum strain, and a method for improving the tolerance of the trichoderma harzianum to the DMI fungicides by using the trichoderma harzianum DMI drug-resistant strain. According to the method, the resistance mutant gene is used for improving the resistance of the trichoderma harzianum to the DMI fungicide, the method can be used for obtaining the DMI fungicide-resistant trichoderma harzianum strain, and the transformant obtained by the method is good in hereditary stability and high in survival fitness and has a good application prospect. 本发明公开了利用抗性突变基因提高哈茨木霉对DMI类杀菌剂耐受性的方法，属于生物技术领域，具体是通过在哈茨木霉菌株中异源表达优化后的禾生球腔菌的抗性突变基因，获得