Kit for realizing coronavirus primary immunization-boosted immunization as well as preparation method and application of kit

The invention provides a kit for realizing coronavirus primary immunization-boosted immunization as well as a preparation method and application of the kit, and relates to the technical field of vaccines. The kit comprises a primary immunization reagent and a booster immunization reagent which are independently packaged respectively, and the primary immunization reagent and the booster immunization reagent are independently selected from novel coronavirus DNA vaccines and coronavirus recombinant DNA vaccines respectively and are DNA vaccines with different components. The novel coronavirus DNA vaccine contains a nucleic acid fragment for coding a new crown SARS-CoV-2 wild type virus spike protein antigen, and the coronavirus recombinant DNA vaccine contains a nucleic acid fragment for coding a SARS-CoV-1 and new crown SARS-CoV-2 Beta mutant strain virus spike protein receptor binding region. According to the kit, a combined immunization scheme of a novel coronavirus DNA vaccine and a coronavirus recombinant D