Aseptic germination and seedling raising method for Maoshan genuine medicinal plant polygonatum sibiricum seeds

The invention discloses a Maoshan genuine medicinal plant Polygonatum sibiricum seed sterile germination seedling raising method, which comprises the following specific steps: collecting immature Polygonatum sibiricum fruits, soaking the fruits with a medicament to rot berry pulp, scrubbing the Poly...

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Bibliographische Detailangaben
Hauptverfasser: GAO DAXIANG, DING JIULING, FANG MINYAN, JIA JUN, SHI JUN, XI GANGJUN, YANG HETONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a Maoshan genuine medicinal plant Polygonatum sibiricum seed sterile germination seedling raising method, which comprises the following specific steps: collecting immature Polygonatum sibiricum fruits, soaking the fruits with a medicament to rot berry pulp, scrubbing the Polygonatum sibiricum seeds, adding a kinetin and gibberellin mixed solution, mixing with river sand, and carrying out low temperature sand storage, the seeds obtained after sand storage treatment are sterilized and disinfected, part of pericarp and endosperm are cut off to expose embryos, the seeds are put into a cluster bud induction culture medium to be subjected to liquid shaking culture to induce cluster buds, finally, the cluster buds are transferred into a seedling strengthening and rooting culture medium to be subjected to seedling strengthening and rooting culture, and the sealwort tissue culture seedlings are formed. According to the method provided by the invention, the immature polygonatum sibiricum embryos