Fluorescent staining solution for vaginal microorganisms and method for detecting vaginal microorganisms

The invention discloses a fluorescent staining solution for vaginal microorganisms and a method for detecting the vaginal microorganisms by using the fluorescent staining solution. The fluorescent staining solution comprises a fluorescent agent, an antibody, an auxiliary dye, a bacteriostatic agent,...

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Hauptverfasser: CAI JINGANG, WU YINGYING, YU JINGJING, GUO YONGCHAO, WANG YANPING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a fluorescent staining solution for vaginal microorganisms and a method for detecting the vaginal microorganisms by using the fluorescent staining solution. The fluorescent staining solution comprises a fluorescent agent, an antibody, an auxiliary dye, a bacteriostatic agent, an anti-quenching agent, a surfactant and a buffer solution, the pH value of the fluorescent staining solution is 4.0-5.0, the fluorescent agent is composed of a first fluorescent agent and a second fluorescent agent, the first fluorescent agent is SYBR Green I or fluorescein isothiocyanate, and the second fluorescent agent is at least one of acridine orange, propidium iodide and a polyrocoxanthin-chlorophyll-protein compound; the antibody is coupled to the second fluorescent agent. According to the present invention, the vaginal microorganism fluorescent staining solution with characteristics of good staining effect and simple operation, and the human vaginal microorganism detection method can be provided. 本公开描述了