Specific real-time fluorescent PCR (Polymerase Chain Reaction) detection primer, probe, method and kit for transgenic potatoes

The invention discloses a real-time fluorescent PCR (Polymerase Chain Reaction) detection primer, a probe, a method and a kit for the specificity of transgenic potatoes. A primer and a TaqMan probe are designed according to a common specific sequence of the innate transgenic potatoes, a real-time fluorescent PCR detection method for the innate transgenic potatoes is established, and the detection primer and the detection probe are utilized to detect according to the method disclosed by the invention to find that the quantitative detection lower limit is 20 copies/mu L, and the detection result shows that the detection result is accurate. Repeatable experiments show that the standard deviation (SD) and the relative standard deviation (RSD) of the method are both within an acceptable range, the specificity is good, the sensitivity is high, the stability is high, and the requirements of supervision departments and the public for strain specificity detection and identification of the innate transgenic potatoes ca