Oocyte cryopreservation antioxidant additive and application

The invention provides an antioxidant additive for improving the cryopreservation effect of oocytes and application, and relates to the technical field of reproductive engineering. The invention provides an antioxidant additive for oocyte cryopreservation. The antioxidant additive comprises an oocyt...

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Bibliographische Detailangaben
Hauptverfasser: ZHOU GUANGBIN, QIN JIANPENG, CAO BEIJIA, PAN BO, WEI YAOZONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides an antioxidant additive for improving the cryopreservation effect of oocytes and application, and relates to the technical field of reproductive engineering. The invention provides an antioxidant additive for oocyte cryopreservation. The antioxidant additive comprises an oocyte vitrification freezing unfreezing solution, an oocyte parthenogenetic activation solution and a parthenogenetic activation embryo in-vitro culture solution, and the oocyte vitrification freezing unfreezing solution, the oocyte parthenogenetic activation solution and the parthenogenetic activation embryo in-vitro culture solution all contain walnut polypeptide TW-7. The oocyte vitrification freezing thawing solution is a sucrose solution containing walnut polypeptide TW-7 and an M2 solution containing the walnut polypeptide TW-7, the concentration of the sucrose solution is 0.5 mol/L, and the concentration of the walnut polypeptide TW-7 is 25 [mu] mol/L. The walnut polypeptide TW-7 with the concentration of 25 [mu