Preparation method of plant growth regulator earthworm polypeptide as well as product and cold-resistant method thereof

The invention discloses a preparation method of a plant growth regulator earthworm polypeptide, which comprises the following steps: step 1, washing earthworms with distilled water, removing silt and wormcast, then draining, soaking with purified water, and draining to obtain a prepared material; st...

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Bibliographische Detailangaben
Hauptverfasser:	DONG HONGMEI, MA ZHIHU, CHEN HAOYU, CUI HAIQIANG, MA BIN, WANG NANXIAO, WANG XUELONG, LI KAI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FORESTRY HORTICULTURE HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF SPIRITS TRAPPING VINEGAR WATERING WINE
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Beschreibung
Zusammenfassung:	The invention discloses a preparation method of a plant growth regulator earthworm polypeptide, which comprises the following steps: step 1, washing earthworms with distilled water, removing silt and wormcast, then draining, soaking with purified water, and draining to obtain a prepared material; step 2, crushing the prepared material into a homogenate state, grinding into paste, adding soybean lecithin, green tea powder and purified water, uniformly mixing to form an emulsified state, carrying out enzymolysis at 30-50 DEG C for 3-5 hours, sieving, removing large particles, then centrifuging, and collecting a centrifugal supernatant; and step 3, dialyzing by adopting a 10000Da dialysis bag, and taking filtrate, namely the earthworm polypeptide autolysis enzymatic hydrolysate. The method has the beneficial effects that the earthworm polypeptide yield and the cold resistance are remarkably improved. The cucumber seedling cold-resistant method based on earthworm polypeptide comprises the following steps: S1, add