PCR (Polymerase Chain Reaction) primer and kit for simultaneously detecting various free banana streak viruses and application of PCR primer and kit

The invention discloses PCR primers and a kit for simultaneously detecting various free banana streak viruses and application of the PCR primers and the kit, and belongs to the technical field of banana virus detection. According to the invention, primer concentrations, annealing temperature conditions and the like in a PCR system are optimized, and the concentrations of upstream and downstream primers of BSOLV, BSGFV and BSIMV are determined to be 0.25 [mu] M/0.25 [mu] M, 0.1 [mu] M/0.1 [mu] M and 0.25 [mu] M/0.25 [mu] M respectively; when the annealing temperature is 56.5 DEG C, the amplification effect is the best. The detection sensitivity of the established multiplex PCR system is 1 * 10 < 3 > copies/mu L, and the established multiplex PCR system has good specificity and can accurately and sensitively detect three different BSV in the compound infection free state in a banana sample. Therefore, the established multiplex PCR is suitable for rapid detection of the three free viruses in banana samples in th