Aromatic alcohol dehydrogenase as well as expression system and application thereof

The invention provides aromatic alcohol dehydrogenase as well as an expression system and application thereof, and belongs to the technical field of molecular biology. The invention provides an aromatic alcohol dehydrogenase sourced from pickled vegetables, the amino acid sequence of the protein of...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: ZHANG WENXUE, WU ZHENGYUN, ZHOU ZIYI, ZHAO YAJIAO
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention provides aromatic alcohol dehydrogenase as well as an expression system and application thereof, and belongs to the technical field of molecular biology. The invention provides an aromatic alcohol dehydrogenase sourced from pickled vegetables, the amino acid sequence of the protein of the aromatic alcohol dehydrogenase is as shown in SEQ ID NO.1, and the nucleotide sequence of the gene is as shown in SEQ ID NO.2. According to the invention, the problem of lack of a new way for producing beta-phenethyl alcohol in the prior art is solved, and an expression system of the aromatic alcohol dehydrogenase is successfully constructed. The protein concentration after induced expression reaches 250 [mu] g/mL, the enzyme activity reaches 15.71 U/mg, and the beta-phenethyl alcohol can be used in the production process of pickled vegetables so as to improve the yield of the key flavor substance beta-phenethyl alcohol of the pickled green vegetables. 本发明提供了一种芳醇脱氢酶及其表达体系和应用,属于分子生物技术领域。本发明提供了一种青菜泡菜来源的芳醇脱氢酶,其蛋白的