Method for improving heme supply level of saccharomyces cerevisiae and application of method

The invention discloses a method for improving the heme supply level of saccharomyces cerevisiae and application of the method, and belongs to the technical field of genetic engineering. According to the invention, the three proteins are positioned in mitochondria through the truncated Hem1p53-549, the truncated Hem14p40-539 and the truncated Hem15p30-393, so that the spatial isolation barrier in the heme synthesis process is removed; a self-assembled structural domain CBD containing a nucleotide sequence as shown in SEQ ID NO.6 is integrated at a cut416d site of a saccharomyces cerevisiae genome, so that intracellular self-assembly of a key enzyme is realized, heme synthesis is improved, and intracellular heme reaches 0.3 mg/L. By expressing soybean hemoglobin, clover hemoglobin, porcine myoglobin, bovine myoglobin, porcine hemoglobin and bovine hemoglobin with functional activity, a foundation is laid for providing intracellular supply for synthesizing protein with heme as a prothetic group in saccharomyces