Polypeptide specifically bound with pendimethalin immune complex and application thereof

The invention discloses a polypeptide specifically bound with a pendimethalin antibody immune complex, and an application of the polypeptide in pendimethalin immunodetection. According to the invention, by using an immune complex formed by a pendimethalin monoclonal antibody and pendimethalin, speci...

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Bibliographische Detailangaben
Hauptverfasser: DING YUAN, MA CHAOFAN, HUANG LIANRUN, WANG YANG, LIANG HONGWU, HUA XIUDE
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a polypeptide specifically bound with a pendimethalin antibody immune complex, and an application of the polypeptide in pendimethalin immunodetection. According to the invention, by using an immune complex formed by a pendimethalin monoclonal antibody and pendimethalin, specifically bound polypeptide is elutriated from a phage display random polypeptide library, and positive clone and a polypeptide sequence thereof are obtained through ELISA and first-generation sequencing. The semi-saturated concentration of the non-competitive phage ELISA established based on the optimal polypeptide is 0.15 ng/mL, which is higher than that of the currently reported pendimethalin immunodetection method. The invention also relates to an application of the polypeptide in a pendimethalin immunochromatography test strip. The visual detection limit of a non-competitive immunochromatography test strip established by the non-phage anti-immune complex polypeptide obtained through chemical synthesis on pendime