In-situ monitoring method for growth state of crops under environmental stress

The invention belongs to the technical field of monitoring, and particularly relates to an electrochemical analysis method for in-situ monitoring of active oxygen generated by crops under environmental stress. According to the invention, a copper picolinate complex Cu (at) PyCA with peroxidase-like activity is combined with reduced graphene with excellent conductivity to form an electrochemical sensor with a 4-Amin/PEDOT: PSS electrode, and crop monitoring is completed through a self-constructed portable detection circuit. The Cu-coated PyCA is adopted to replace biological enzyme, and the constructed electrochemical sensor is high in stability, high in catalytic activity and low in cost; in addition, by means of the biocompatibility of the conductive hydrogel, in-situ monitoring of hydrogen peroxide can be directly carried out without liquid electrolyte, and accurate tracking of active oxygen in the growth process of crops is achieved. 本发明属于监测技术领域，具体涉及一种环境胁迫下农作物产生活性氧原位监测的电化学分析方法。本发明将具有类过氧化物酶活性的吡甲酸铜络合物Cu@PyCA