Pig rotavirus detection primer composition, kit and detection method

The invention discloses a porcine rotavirus detection primer composition, and detection primers comprise an upstream primer SEQ ID NO.1, a downstream primer SEQ ID NO.2 and a fluorescent probe SEQ ID NO.3. According to the detection method provided by the invention, specific primers and a kit are ad...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	ZHANG RONGCAI, YANG ZHENTAN, QIAN HONGYUN, ZENG FANHUANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses a porcine rotavirus detection primer composition, and detection primers comprise an upstream primer SEQ ID NO.1, a downstream primer SEQ ID NO.2 and a fluorescent probe SEQ ID NO.3. According to the detection method provided by the invention, specific primers and a kit are adopted to prepare a PCR reaction liquid to establish a porcine rotavirus fluorescent quantitative PCR rapid detection method, and the detection method has the characteristics of short detection process time, high sensitivity, high sensitivity and the like. The applicability is wide, and the operation is simple and convenient. 本发明公开一种猪轮状病毒检测引物组合物，其中检测引物包括上游引物SEQ ID NO.1、下游引物SEQ ID NO.2和荧光探针SEQ ID NO.3，本发明的检测方法采用特异性引物及试剂盒配置形成PCR反应液建立的猪轮状病毒荧光定量PCR快速检测方法，本发明具有检测流程时间短，适用性广，操作简便的优点。