Medium and method for culturing small intestine organoid of sheep family

The invention discloses a sheep small intestine organoid culture medium. The sheep small intestine organoid culture medium is prepared by adding an N2 supplement, a B27 serum substitute, N-acetylcysteine, nicotinamide, A83-01, SB202190, Y27632, HEPES, GlutaMax, a mycillin mixed solution, a Wnt3a, an...

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Bibliographische Detailangaben
Hauptverfasser:	HUANG ZAN, ZHU WEIYUN, LIU JUNHUA, ZHU HAINING, MAO SHENGYONG, QIN JING, SONG MINGMEI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a sheep small intestine organoid culture medium. The sheep small intestine organoid culture medium is prepared by adding an N2 supplement, a B27 serum substitute, N-acetylcysteine, nicotinamide, A83-01, SB202190, Y27632, HEPES, GlutaMax, a mycillin mixed solution, a Wnt3a, an R-spondin1 conditioned culture medium, Noggin and EGF (Epidermal Growth Factor) into an Advanced DMEM (Dulbecco Modified Eagle Medium)/F12 culture medium. Meanwhile, the invention discloses a method for culturing by using the sheep small intestine organoid culture medium. The culture medium disclosed by the invention can promote stem cells in each intestinal segment crypt of the small intestine of the sheep family to proliferate and differentiate so as to form organoids in vitro; and the method also has the advantages of few used growth factors, low cost and high consistency. 本发明公开了一种羊科小肠类器官培养基，在Advanced DMEM/F12培养基中添加N2补充剂、B27血清替代物、N-乙酰半胱氨酸、烟酰胺、A83-01、SB202190、Y27632、HEPES、GlutaMax、青链霉素混合液、Wnt3a、R-spondin1条件培养基、N