Application of peripheral blood antigen specific CX3CR1 + T cell and culture method of peripheral blood antigen specific CX3CR1 + T cell

The invention belongs to the technical field of immune cells and immunotherapy, and particularly relates to application and a culture method of a peripheral blood antigen specific CX3CR1 + T cell subset. According to the invention, single cell transcriptome and TCR sequencing is carried out on paired peripheral blood and tumor tissue specimens in a lung cancer patient, and it is found that the CX3CR1 + T cell has high similarity with TIL TCR and has an anti-tumor effect characteristic; furthermore, a clinical lung cancer patient sample is collected, the expression level and function of CX3CR1 in peripheral blood of the clinical lung cancer patient sample are comprehensively tested and explored, it is proved for the first time that the CX3CR1 + T cell subset has the anti-tumor effect and belongs to immune cells with antigen specificity and high functions in the peripheral blood, and the CX3CR1 + T cell subset has the anti-tumor effect. Therefore, the CX3CR1 + T cell is a novel cell subset with tumor killing ab