Method for detecting related substances of compound permethrin and pyriproxyfen preparations

The invention provides a method for detecting related substances in compound permethrin and pyriproxyfen preparations, and belongs to the technical field of pharmaceutical analysis. The detection method comprises the following steps: detecting related substances in compound permethrin, pyriproxyfen and fipronil preparations by adopting a reversed-phase high-performance liquid chromatography, sequentially preparing a test solution and a contrast solution, respectively injecting the test solution and the contrast solution into a high-performance liquid chromatograph, taking an octadecylsilane bonded silica gel column as a chromatographic column, and detecting the content of the related substances in the compound permethrin, pyriproxyfen and fipronil preparations. An ammonium acetate buffer solution is used as a mobile phase A, an acetonitrile-methanol mixed solution is used as a mobile phase B, and gradient elution is carried out. The method disclosed by the invention is strong in specificity and good in repeat