Method for detecting activity of branched chain amino acid aminotransferase and application
The invention belongs to the technical field of enzyme activity detection, and particularly relates to a method for detecting the activity of branched chain amino acid aminotransferase and application. The method comprises the following steps: adding branched chain amino acid covalently linked by ca...
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| creator | SHI ZHAN CUI HUINA WANG JIAOJIAO WEI QINGYAN SHI FEI LI YAN HE YUN WEN HE LIU LI |
| description | The invention belongs to the technical field of enzyme activity detection, and particularly relates to a method for detecting the activity of branched chain amino acid aminotransferase and application. The method comprises the following steps: adding branched chain amino acid covalently linked by carbon 13 and nitrogen 15, alpha-ketoglutaric acid and branched chain amino acid aminotransferase into a reaction buffer solution to obtain a sample to be detected, and performing one-dimensional HNCA nuclear magnetic resonance detection to obtain the area of a one-dimensional HNCA hydrogen spectrum signal peak; the method comprises the following steps: preparing a plurality of branched chain amino acids covalently linked with carbon 13 and nitrogen 15 with known concentrations, carrying out one-dimensional HNCA nuclear magnetic resonance detection to obtain the area of a one-dimensional HNCA hydrogen spectrum signal peak, and obtaining a standard curve according to the area and the known concentrations; and obtainin |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | Method for detecting activity of branched chain amino acid aminotransferase and application |
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