Method for detecting activity of branched chain amino acid aminotransferase and application

The invention belongs to the technical field of enzyme activity detection, and particularly relates to a method for detecting the activity of branched chain amino acid aminotransferase and application. The method comprises the following steps: adding branched chain amino acid covalently linked by carbon 13 and nitrogen 15, alpha-ketoglutaric acid and branched chain amino acid aminotransferase into a reaction buffer solution to obtain a sample to be detected, and performing one-dimensional HNCA nuclear magnetic resonance detection to obtain the area of a one-dimensional HNCA hydrogen spectrum signal peak; the method comprises the following steps: preparing a plurality of branched chain amino acids covalently linked with carbon 13 and nitrogen 15 with known concentrations, carrying out one-dimensional HNCA nuclear magnetic resonance detection to obtain the area of a one-dimensional HNCA hydrogen spectrum signal peak, and obtaining a standard curve according to the area and the known concentrations; and obtainin