Method and kit for rapidly, simply and conveniently detecting c.235delC mutation site of GJB2 deafness gene
The invention discloses a method and a kit for rapidly, simply and conveniently detecting a c.235delC mutation site of a GJB2 deafness gene, and the method comprises the following steps: by taking DNA of a sample to be detected as a template, carrying out RAA amplification by adopting a primer pair...
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| creator | DENG YUEQIN WANG WEI LIU ZIDI HE YIHONG YANG MING XU JUAN YANG YIFANG |
| description | The invention discloses a method and a kit for rapidly, simply and conveniently detecting a c.235delC mutation site of a GJB2 deafness gene, and the method comprises the following steps: by taking DNA of a sample to be detected as a template, carrying out RAA amplification by adopting a primer pair consisting of a sequence shown as a sequence 1 and a sequence shown as a sequence 2 to obtain an RAA amplification product; meanwhile, replacing the DNA template of the to-be-detected sample with enzyme-free water as a negative control; the method comprises the following steps: preparing a CRISPR-Cas13a detection system, and then carrying out a cutting report RNA reaction; the CRISPR-Cas13a detection system comprises the RAA amplification product, a CRISPR-Cas13a system, test paper report RNA (Ribonucleic Acid) as shown in a sequence 7, NTP (Nitrophenyl Thiophosphate), T7RNA polymerase and an RNA enzyme inhibitor; dropwise adding the reaction liquid obtained after the report RNA is cut by Cas13a in the step 2 into |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method and kit for rapidly, simply and conveniently detecting c.235delC mutation site of GJB2 deafness gene |
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