Method and kit for rapidly, simply and conveniently detecting c.235delC mutation site of GJB2 deafness gene

The invention discloses a method and a kit for rapidly, simply and conveniently detecting a c.235delC mutation site of a GJB2 deafness gene, and the method comprises the following steps: by taking DNA of a sample to be detected as a template, carrying out RAA amplification by adopting a primer pair consisting of a sequence shown as a sequence 1 and a sequence shown as a sequence 2 to obtain an RAA amplification product; meanwhile, replacing the DNA template of the to-be-detected sample with enzyme-free water as a negative control; the method comprises the following steps: preparing a CRISPR-Cas13a detection system, and then carrying out a cutting report RNA reaction; the CRISPR-Cas13a detection system comprises the RAA amplification product, a CRISPR-Cas13a system, test paper report RNA (Ribonucleic Acid) as shown in a sequence 7, NTP (Nitrophenyl Thiophosphate), T7RNA polymerase and an RNA enzyme inhibitor; dropwise adding the reaction liquid obtained after the report RNA is cut by Cas13a in the step 2 into