Chromatographic separation and purification method of silk fibroin

The invention provides a silk fibroin chromatographic separation and purification method which comprises the following steps: uniformly mixing a silk fibroin raw material, a sodium chloride solution and ethanol to obtain a silk fibroin solution; adjusting the pH value of the obtained silk fibroin solution to 8-10, taking the silk fibroin solution as an upper column solution, adsorbing by adopting a chromatographic column, and eluting by adopting an aqueous solution of glycine and urea to obtain an eluent; and adjusting the pH value of the obtained eluent to 2-5, precipitating the silk fibroin, and filtering to obtain the silk fibroin. According to the chromatographic separation and purification method of the silk fibroin, the chromatographic column is not easy to block, and the product yield is relatively high. 本发明提供一种丝素蛋白的层析分离纯化方法，其包括如下步骤：取丝素蛋白原料、氯化钠溶液、乙醇，混合均匀，得到丝素蛋白溶液；将得到的所述丝素蛋白溶液调节pH值至8-10，作为上柱液，采用层析柱吸附，再采用甘氨酸与尿素的水溶液进行洗脱，得到洗脱液；将得到的洗脱液的pH值调节至2-5，使所述丝素蛋白沉淀，过滤后，得到丝素蛋白。本发明所述的丝素蛋白的层析分离纯化方法，不易堵塞层析柱，且产品收率较高。