Preparation method of picrorhiza multicomponent compound

The invention belongs to the technical field of traditional Chinese medicines, and particularly discloses a separation and preparation method of picrorhiza rhizome multi-component compounds. Comprising the following steps: loading a rhizoma picrorhizae crude extract sample on a dynamic axial compression column, carrying out gradient or isocratic elution with ethyl acetate-absolute ethyl alcohol to obtain a rhizoma picrorhizae mixture crude product, carrying out gradient elution separation with a purified water-acetonitrile solution, carrying out online detection with an ultraviolet detector, and collecting an effluent to obtain a purified product of each component compound. The method disclosed by the invention is simple to operate and short in period, and the obtained product is relatively high in purity and strong in controllability, can be used as a standard substance, and has important guiding significance on chemical component research and quality control of the rhizoma picrorhizae extract. 本发明属于中药技术领域，具