Method for inducing gardenia jasminoides embryonic callus with high crocin content

The invention provides an induction method of fructus gardeniae embryogenic callus with high crocin content. The method comprises the following steps: screening high-yield fructus gardeniae with high crocin content as a culture material, and carrying out induced culture on leaves of the fructus gard...

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Hauptverfasser: DENG SHAOYONG, HU SHAN, GAO LIQIN, FENG XIAOTAO, YU LIXIN, ZHU LINGZHI, LI KANGQIN, WANG MEIXIA, CHEN YIJUN, YANG CHUNXIA, XIE XIONGXIONG, HU XIAOHONG, HUANG JING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides an induction method of fructus gardeniae embryogenic callus with high crocin content. The method comprises the following steps: screening high-yield fructus gardeniae with high crocin content as a culture material, and carrying out induced culture on leaves of the fructus gardeniae to successfully induce calluses with the induction rate of 100%; the calluses induced by the method are green granules, the problems of brown, whitening and the like do not appear in subsequent proliferation experiments, and adventitious buds can be successfully induced; according to the method provided by the invention, the yield and quality of the embryogenic callus are improved, the embryogenic callus is normal in subculture proliferation, and good embryogenic property is kept; the somatic embryo seedlings obtained through the technology can be directly applied to industrial production and have good social and economic benefits. 本发明提供了一种高西红花苷含量水栀子胚性愈伤组织的诱导方法;通过筛选出产量高的高西红花苷含量的水栀子作为培育材料,将其叶片进行诱导培养,成功诱导出愈伤组织,