Method for rapidly detecting sheep FecB gene single nucleotide polymorphism by using KASP and application thereof

The invention discloses a method for rapidly detecting single nucleotide polymorphism of a sheep FecB (also being BMPR1B) gene by using KASP and application of the method. According to the present invention, sheep whole genome DNA is adopted as a template, accurate typing is performed on an SNP site derived from a sheep FecB gene through a KASP method, association analysis discovers that different genotypes of the SNP site are significantly related to the litter size trait of a certain birth of sheep, and the molecular marker exists as the early selection for improving the breeding trait of the sheep; by rapidly and accurately detecting the SNP marker related to the lambing number character of the sheep, a sheep breeding dominant genetic resource group can be rapidly established, so that the marker-assisted selection breeding process of the sheep breeding character is accelerated. 本发明公开了一种利用KASP快速检测绵羊FecB(又被为BMPR1B)基因单核苷酸多态性的方法及其应用。以绵羊全基因组DNA为模板，通过KASP方法对来源于绵羊FecB基因的一个SNP位点进行准确分型，关联分析发现该SNP位点的不同基因型与绵羊某胎次产羔数性状