SNP (Single Nucleotide Polymorphism) molecular marker for identifying hereditary sex of Qinglongfish and application thereof
The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying hereditary sex of Qinglongfish and application of the SNP molecular marker. Belongs to the technical field of fish sex identification. The nucleotide sequence of the molecular marker is as shown in SEQ I...
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Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying hereditary sex of Qinglongfish and application of the SNP molecular marker. Belongs to the technical field of fish sex identification. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO.1, the molecular marker comprises an SNP (Single Nucleotide Polymorphism) site, and the SNP site is positioned at the 192nd basic group at the 5'end of the molecular marker. Single nucleotide polymorphisms (SNP) which have differences between male and female green dragon fish individuals and can characterize sex are obtained through genome high-throughput sequencing comparison, an amplification sequence containing the SNP sites is obtained by combining primer amplification, on the basis, PCR amplification and sequencing of a to-be-detected sample are achieved through primers, sample SNP site information is obtained, and the SNP site information of the to-be-detected sample is compared with the SNP site informa |
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