Preparation method and preservation method of pleuromutilin strain
The invention relates to a preparation method and a preservation method of a pleuromutilin strain. The preparation method of the pleuromutilin strain comprises the following steps: (1) inoculating a high-yield strain onto a culture medium, preparing the high-yield strain into a bacterial suspension...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | LIU XINGGUO ZHAO MING HE YUYING QIU SHUILAN CHANG ZHANHUA |
| description | The invention relates to a preparation method and a preservation method of a pleuromutilin strain. The preparation method of the pleuromutilin strain comprises the following steps: (1) inoculating a high-yield strain onto a culture medium, preparing the high-yield strain into a bacterial suspension containing mycelium fragments, and diluting the bacterial suspension; (2) coating a plate with the diluent, and carrying out plate culture; (3) selecting single colonies according to morphological characteristics of the single colonies, and inoculating the single colonies to the inclined plane; (4) slant culture; (5) selecting bacterial colonies according to morphological characteristics of mature slant strains; and (6) carrying out a strain sterile titer test on the bacterial colony selected in the step (5), and selecting a high-titer strain, namely the pleuromutilin strain. According to the preparation method and the preservation method of the pleuromutilin strain, the screened strain is high in breeding speed, g |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN117327764A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN117327764A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN117327764A3</originalsourceid><addsrcrecordid>eNrjZHAKKEotSCxKLMnMz1PITS3JyE9RSMxLUSgoSi1OLSpDEc9PUyjISS0tys8tLcnMycxTKC4pSszM42FgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFicmpeakl8c5-hobmxkbm5mYmjsbEqAEAovQy8A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Preparation method and preservation method of pleuromutilin strain</title><source>esp@cenet</source><creator>LIU XINGGUO ; ZHAO MING ; HE YUYING ; QIU SHUILAN ; CHANG ZHANHUA</creator><creatorcontrib>LIU XINGGUO ; ZHAO MING ; HE YUYING ; QIU SHUILAN ; CHANG ZHANHUA</creatorcontrib><description>The invention relates to a preparation method and a preservation method of a pleuromutilin strain. The preparation method of the pleuromutilin strain comprises the following steps: (1) inoculating a high-yield strain onto a culture medium, preparing the high-yield strain into a bacterial suspension containing mycelium fragments, and diluting the bacterial suspension; (2) coating a plate with the diluent, and carrying out plate culture; (3) selecting single colonies according to morphological characteristics of the single colonies, and inoculating the single colonies to the inclined plane; (4) slant culture; (5) selecting bacterial colonies according to morphological characteristics of mature slant strains; and (6) carrying out a strain sterile titer test on the bacterial colony selected in the step (5), and selecting a high-titer strain, namely the pleuromutilin strain. According to the preparation method and the preservation method of the pleuromutilin strain, the screened strain is high in breeding speed, g</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240102&DB=EPODOC&CC=CN&NR=117327764A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25568,76551</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240102&DB=EPODOC&CC=CN&NR=117327764A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU XINGGUO</creatorcontrib><creatorcontrib>ZHAO MING</creatorcontrib><creatorcontrib>HE YUYING</creatorcontrib><creatorcontrib>QIU SHUILAN</creatorcontrib><creatorcontrib>CHANG ZHANHUA</creatorcontrib><title>Preparation method and preservation method of pleuromutilin strain</title><description>The invention relates to a preparation method and a preservation method of a pleuromutilin strain. The preparation method of the pleuromutilin strain comprises the following steps: (1) inoculating a high-yield strain onto a culture medium, preparing the high-yield strain into a bacterial suspension containing mycelium fragments, and diluting the bacterial suspension; (2) coating a plate with the diluent, and carrying out plate culture; (3) selecting single colonies according to morphological characteristics of the single colonies, and inoculating the single colonies to the inclined plane; (4) slant culture; (5) selecting bacterial colonies according to morphological characteristics of mature slant strains; and (6) carrying out a strain sterile titer test on the bacterial colony selected in the step (5), and selecting a high-titer strain, namely the pleuromutilin strain. According to the preparation method and the preservation method of the pleuromutilin strain, the screened strain is high in breeding speed, g</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHAKKEotSCxKLMnMz1PITS3JyE9RSMxLUSgoSi1OLSpDEc9PUyjISS0tys8tLcnMycxTKC4pSszM42FgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFicmpeakl8c5-hobmxkbm5mYmjsbEqAEAovQy8A</recordid><startdate>20240102</startdate><enddate>20240102</enddate><creator>LIU XINGGUO</creator><creator>ZHAO MING</creator><creator>HE YUYING</creator><creator>QIU SHUILAN</creator><creator>CHANG ZHANHUA</creator><scope>EVB</scope></search><sort><creationdate>20240102</creationdate><title>Preparation method and preservation method of pleuromutilin strain</title><author>LIU XINGGUO ; ZHAO MING ; HE YUYING ; QIU SHUILAN ; CHANG ZHANHUA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117327764A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIU XINGGUO</creatorcontrib><creatorcontrib>ZHAO MING</creatorcontrib><creatorcontrib>HE YUYING</creatorcontrib><creatorcontrib>QIU SHUILAN</creatorcontrib><creatorcontrib>CHANG ZHANHUA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIU XINGGUO</au><au>ZHAO MING</au><au>HE YUYING</au><au>QIU SHUILAN</au><au>CHANG ZHANHUA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Preparation method and preservation method of pleuromutilin strain</title><date>2024-01-02</date><risdate>2024</risdate><abstract>The invention relates to a preparation method and a preservation method of a pleuromutilin strain. The preparation method of the pleuromutilin strain comprises the following steps: (1) inoculating a high-yield strain onto a culture medium, preparing the high-yield strain into a bacterial suspension containing mycelium fragments, and diluting the bacterial suspension; (2) coating a plate with the diluent, and carrying out plate culture; (3) selecting single colonies according to morphological characteristics of the single colonies, and inoculating the single colonies to the inclined plane; (4) slant culture; (5) selecting bacterial colonies according to morphological characteristics of mature slant strains; and (6) carrying out a strain sterile titer test on the bacterial colony selected in the step (5), and selecting a high-titer strain, namely the pleuromutilin strain. According to the preparation method and the preservation method of the pleuromutilin strain, the screened strain is high in breeding speed, g</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN117327764A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Preparation method and preservation method of pleuromutilin strain |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T06%3A27%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=LIU%20XINGGUO&rft.date=2024-01-02&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN117327764A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |