Preparation method and preservation method of pleuromutilin strain

The invention relates to a preparation method and a preservation method of a pleuromutilin strain. The preparation method of the pleuromutilin strain comprises the following steps: (1) inoculating a high-yield strain onto a culture medium, preparing the high-yield strain into a bacterial suspension containing mycelium fragments, and diluting the bacterial suspension; (2) coating a plate with the diluent, and carrying out plate culture; (3) selecting single colonies according to morphological characteristics of the single colonies, and inoculating the single colonies to the inclined plane; (4) slant culture; (5) selecting bacterial colonies according to morphological characteristics of mature slant strains; and (6) carrying out a strain sterile titer test on the bacterial colony selected in the step (5), and selecting a high-titer strain, namely the pleuromutilin strain. According to the preparation method and the preservation method of the pleuromutilin strain, the screened strain is high in breeding speed, g