Three-dimensional bionic oriented gel scaffold as well as preparation method and application thereof

The invention provides a three-dimensional bionic oriented gel scaffold as well as a preparation method and application thereof, and the preparation method comprises the following steps: preparing a phase separation mixed solution which comprises B-type gelatin with the final concentration of 4% and konjac glucomannan with the final concentration of 0.1-0.5%; mixing a cross-linking agent with the phase separation mixed solution, and performing cross-linking gelation to obtain a third precursor; the third precursor is subjected to directional freezing and freeze drying at the temperature ranging from-20 DEG C to-80 DEG C, the three-dimensional bionic oriented gel scaffold is obtained, and the prepared gel scaffold has good biocompatibility and degradability, has edibility and has huge potential in the aspects of muscle fiber tissue repair and regeneration. 本发明提供一种三维仿生取向凝胶支架及其制备方法及应用，制备相分离混合溶液，其中包含终浓度为4％的B型明胶和终浓度为0.1％～0.5％魔芋葡甘聚糖；将交联剂与相分离混合溶液混合后进行交联凝胶化，得到第三前驱体；对第三前驱体在-20℃～-80℃进行定向冷冻、冷冻干燥，得到三维仿生取向凝胶支架，制得的凝胶支架具有良好