Data quality control method and microorganism identification method based on first-generation sequencing technology
The invention belongs to the field of biological information, and particularly relates to a data quality control method and a microbiological identification method based on a first-generation sequencing technology, and the quality control method comprises the following steps: obtaining all file path...
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Zusammenfassung: | The invention belongs to the field of biological information, and particularly relates to a data quality control method and a microbiological identification method based on a first-generation sequencing technology, and the quality control method comprises the following steps: obtaining all file paths with a format of ab1 in a specified folder of a first-generation sequencing result, and setting circulation for one-by-one processing; reading a single ab1 file to obtain sequencing quality information, and intercepting qualified fragments according to a threshold sliding window; circularly processing each qualified fragment, removing fragments with mixed signals, and selecting the fragment with the maximum length from the residual fragments; and finally, judging whether the length of the fragment with the maximum length in the result accords with a quality control threshold value or not. If yes, quality control succeeds; if the quality control fails, the sequencing result is unqualified, and the process is termi |
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