Urease detection kit

The invention discloses a urease detection kit which comprises a urease detection reagent and a color developing solution containing a color developing agent, and a substrate of the urease detection reagent is urea or a urea derivative; and the color developing agent is any one of 4-dimethylamino ci...

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Hauptverfasser: YANG YONGKANG, SONG SHAOYING, YANG SEN, LIU ZHILEI, LIU YAFENG, LI XIAOXIA, WANG LIQIN, FU GUANGYU, WANG ZEYU, YANG ZENGLI, YANG HONGYUN, WANG LIYING, HU YU, ZHANG XUEDONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a urease detection kit which comprises a urease detection reagent and a color developing solution containing a color developing agent, and a substrate of the urease detection reagent is urea or a urea derivative; and the color developing agent is any one of 4-dimethylamino cinnamyl aldehyde and a derivative of 4-dimethylamino cinnamyl aldehyde. According to the method, urea or derivatives thereof (such as hydroxymethyl urea and urea phosphate) are taken as reaction substrates of urease, and the principle that 4-dimethylaminocinnamaldehyde can be subjected to chromogenic reaction with urea is utilized to judge whether urease exists or not, so that the method is completely not influenced by the pH value of a sample, the accuracy is improved, misjudgment is reduced, and the detection accuracy is improved. The method overcomes the technical defect that the existing detection method is easy to misjudge when the pH change is taken as a judgment standard. 本发明公开了一种脲酶检测试剂盒,包括脲酶检测试剂和含有显色剂的显色液,脲酶