Detection method of dioscin ketone

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a detection method of dioscin ketone. The detection method comprises the following steps: acquiring a detection wavelength, preparing a standard substance, and drawing a standard curve by using the standard substance. According to the present invention, the detection wavelength of dioscin ketone is determined to be 254 nm; in addition, according to the invention, the matching condition of the chromatographic column eluent for detecting dioscin is defined as follows: the ratio of methanol to water is 90: 10. According to the method, diosgenin and dioscin which are highly similar in molecular structure can be distinguished on the premise that dioscin is not damaged, detection is carried out through high performance liquid chromatography, and confusion of the diosgenin and the dioscin is avoided. The detection method provided by the invention is high in sensitivity and low in detection cost, and provides a refere