Macroporous chitosan beads and preparation method thereof

本发明涉及大孔脱乙酰壳多糖小珠，它具有大小为5-200μm的孔，这些孔相对较大且均匀分布到小珠表面和核区，以及所述小珠的制备方法包括下列步骤：将脱乙酰壳多糖溶液、脱乙酰壳多糖水溶液或它们的混合物滴加到低温有机溶剂或液氮中；通过由温度差异所致的相分离法调整孔径大小。本发明的大孔脱乙酰壳多糖小珠比现有基体使细胞培养更有效，因为由于这些小珠具有较大表面积而使细胞能够有效地附着其上，由于这些小珠的三维结构，注射到小珠中的细胞和附着于小珠的细胞能够容易地长期存在，因此它们能够用于研究生产蛋白质、抗生素、抗癌物质、多糖、生理活性物质、动物激素、或植物激素，以及用于研究代谢器官，软骨或骨的替代品。 The present invention relates to macroporous chitosan beads having 5-200 mum in size of relatively large and uniform pores that are distributed from surface to core region, and a preparation method thereof compring the following steps; by dropping chitosan solution, aqueous chitosan solution or their mixture into the low-temperature of organic solvent or liquid nitrogen; and by regulating pore size by phase separation method via temperature difference. The macroporous chitosan beads of the present invention make cell culture more efficient than the previous substrate, since cell can attach to them efficiently due to their large surface area, it is easy for cell to be injected into them and cell attached to them can exist longer due to their three-dimensional structure, therefore they can be used for a study about production of protein, abtibiotics, anticancer agent, polysaccharide, physiologically active agent, animal hormone, or plant hormone as well as a study about substitution of metabolic organs, cartilage or bone.