Colorimetric detection method for food-borne pathogenic bacteria based on Fe3O4-coated MIL-100 (Fe) magnetic separation and nano-enzyme catalysis
The invention discloses a colorimetric detection method for food-borne pathogenic bacteria based on magnetic separation and nano-enzyme catalysis of Fe3O4 (at) MIL-100 (Fe). The colorimetric detection method mainly comprises the following steps: preparing a Fe3O4 (at) MIL-100 (Fe) material by a solv...
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| creator | LI JUNGUANG DU JUAN XIANG QISEN GUO JIANGLI BAI YANHONG LI KE ZHAO DIANBO LIU KAI LI ZONGSHUANG |
| description | The invention discloses a colorimetric detection method for food-borne pathogenic bacteria based on magnetic separation and nano-enzyme catalysis of Fe3O4 (at) MIL-100 (Fe). The colorimetric detection method mainly comprises the following steps: preparing a Fe3O4 (at) MIL-100 (Fe) material by a solvothermal method and an in-situ growth method, modifying the material by using an aptamer to prepare a Fe3O4 (at) MIL-100 (Fe) aptamer, and realizing capture and magnetic separation of the food-borne pathogenic bacteria. The food-borne pathogenic bacteria are rapidly detected by using the nano-enzyme catalytic activity of the Fe3O4-MIL-100 (Fe) aptamer and using TMB as a substrate to catalyze and generate a product. The method disclosed by the invention has the advantages of high detection sensitivity, strong specificity, short time consumption and the like, and is suitable for rapid detection of food-borne pathogenic bacteria in food samples.
本发明公开了一种基于Fe3O4@MIL-100(Fe)磁分离和纳米酶催化的食源性致病菌比色检测方法：主要是先通过溶剂热法及原位生长法制备Fe3O4 |
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本发明公开了一种基于Fe3O4@MIL-100(Fe)磁分离和纳米酶催化的食源性致病菌比色检测方法：主要是先通过溶剂热法及原位生长法制备Fe3O4</description><language>chi ; eng</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231222&DB=EPODOC&CC=CN&NR=117269490A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25568,76551</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231222&DB=EPODOC&CC=CN&NR=117269490A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LI JUNGUANG</creatorcontrib><creatorcontrib>DU JUAN</creatorcontrib><creatorcontrib>XIANG QISEN</creatorcontrib><creatorcontrib>GUO JIANGLI</creatorcontrib><creatorcontrib>BAI YANHONG</creatorcontrib><creatorcontrib>LI KE</creatorcontrib><creatorcontrib>ZHAO DIANBO</creatorcontrib><creatorcontrib>LIU KAI</creatorcontrib><creatorcontrib>LI ZONGSHUANG</creatorcontrib><title>Colorimetric detection method for food-borne pathogenic bacteria based on Fe3O4-coated MIL-100 (Fe) magnetic separation and nano-enzyme catalysis</title><description>The invention discloses a colorimetric detection method for food-borne pathogenic bacteria based on magnetic separation and nano-enzyme catalysis of Fe3O4 (at) MIL-100 (Fe). The colorimetric detection method mainly comprises the following steps: preparing a Fe3O4 (at) MIL-100 (Fe) material by a solvothermal method and an in-situ growth method, modifying the material by using an aptamer to prepare a Fe3O4 (at) MIL-100 (Fe) aptamer, and realizing capture and magnetic separation of the food-borne pathogenic bacteria. The food-borne pathogenic bacteria are rapidly detected by using the nano-enzyme catalytic activity of the Fe3O4-MIL-100 (Fe) aptamer and using TMB as a substrate to catalyze and generate a product. The method disclosed by the invention has the advantages of high detection sensitivity, strong specificity, short time consumption and the like, and is suitable for rapid detection of food-borne pathogenic bacteria in food samples.
本发明公开了一种基于Fe3O4@MIL-100(Fe)磁分离和纳米酶催化的食源性致病菌比色检测方法：主要是先通过溶剂热法及原位生长法制备Fe3O4</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjbFuAjEQRK-hiAL_sHSksHQXTiDK6JQTSAlp0qPFngNLd17LdgN_wR9nFeUDUqxm52lm96l6dDJK8hNK8pYcCmzxEkjBVRwNknTEmbOkAIqs9IKg0TPbguRZlwxHWumx_mqNFS7qPw8fpqlrWvV4oYkvAUVLGZET_z7g4ChwEINwv00gy4XHW_Z5Xs0GHjMWf_pcLfv3725vEOWEHNlCj526Y9NsXze7dle_rf-T-QHESU2m</recordid><startdate>20231222</startdate><enddate>20231222</enddate><creator>LI JUNGUANG</creator><creator>DU JUAN</creator><creator>XIANG QISEN</creator><creator>GUO JIANGLI</creator><creator>BAI YANHONG</creator><creator>LI KE</creator><creator>ZHAO DIANBO</creator><creator>LIU KAI</creator><creator>LI ZONGSHUANG</creator><scope>EVB</scope></search><sort><creationdate>20231222</creationdate><title>Colorimetric detection method for food-borne pathogenic bacteria based on Fe3O4-coated MIL-100 (Fe) magnetic separation and nano-enzyme catalysis</title><author>LI JUNGUANG ; DU JUAN ; XIANG QISEN ; GUO JIANGLI ; BAI YANHONG ; LI KE ; ZHAO DIANBO ; LIU KAI ; LI ZONGSHUANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117269490A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>LI JUNGUANG</creatorcontrib><creatorcontrib>DU JUAN</creatorcontrib><creatorcontrib>XIANG QISEN</creatorcontrib><creatorcontrib>GUO JIANGLI</creatorcontrib><creatorcontrib>BAI YANHONG</creatorcontrib><creatorcontrib>LI KE</creatorcontrib><creatorcontrib>ZHAO DIANBO</creatorcontrib><creatorcontrib>LIU KAI</creatorcontrib><creatorcontrib>LI ZONGSHUANG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LI JUNGUANG</au><au>DU JUAN</au><au>XIANG QISEN</au><au>GUO JIANGLI</au><au>BAI YANHONG</au><au>LI KE</au><au>ZHAO DIANBO</au><au>LIU KAI</au><au>LI ZONGSHUANG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Colorimetric detection method for food-borne pathogenic bacteria based on Fe3O4-coated MIL-100 (Fe) magnetic separation and nano-enzyme catalysis</title><date>2023-12-22</date><risdate>2023</risdate><abstract>The invention discloses a colorimetric detection method for food-borne pathogenic bacteria based on magnetic separation and nano-enzyme catalysis of Fe3O4 (at) MIL-100 (Fe). The colorimetric detection method mainly comprises the following steps: preparing a Fe3O4 (at) MIL-100 (Fe) material by a solvothermal method and an in-situ growth method, modifying the material by using an aptamer to prepare a Fe3O4 (at) MIL-100 (Fe) aptamer, and realizing capture and magnetic separation of the food-borne pathogenic bacteria. The food-borne pathogenic bacteria are rapidly detected by using the nano-enzyme catalytic activity of the Fe3O4-MIL-100 (Fe) aptamer and using TMB as a substrate to catalyze and generate a product. The method disclosed by the invention has the advantages of high detection sensitivity, strong specificity, short time consumption and the like, and is suitable for rapid detection of food-borne pathogenic bacteria in food samples.
本发明公开了一种基于Fe3O4@MIL-100(Fe)磁分离和纳米酶催化的食源性致病菌比色检测方法：主要是先通过溶剂热法及原位生长法制备Fe3O4</abstract><oa>free_for_read</oa></addata></record> |
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| title | Colorimetric detection method for food-borne pathogenic bacteria based on Fe3O4-coated MIL-100 (Fe) magnetic separation and nano-enzyme catalysis |
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