Establishment method and application of climacterium HPLC (High Performance Liquid Chromatography) fingerprint spectrum

The invention relates to a construction method and application of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of climacteric cinnabine, which can comprehensively evaluate and control the quality of climacteric cinnabine, is beneficial to stabilizing the product quality, ensures the stability and uniformity of the internal quality of the climacteric cinnabine and ensures the safety and effectiveness of clinical medication. The construction method of the Qiannigning HPLC fingerprint spectrum comprises the following steps: 1) preparing a reference substance solution; 2) preparing a medicinal material solution; (3) preparing a test solution; 4) determination of HPLC chromatographic conditions; 5) calculating and determining the ratio of the relative retention time to the relative peak area of nine main characteristic peaks with relatively large peak areas; the method disclosed by the invention is simple, convenient and rapid to operate, good in reproducibility and high in stability, can