Method for detecting penthiopyrad and metabolite thereof in animal-derived food

The invention discloses a method for detecting penthiopyrad and metabolite thereof in animal-derived food, which comprises the following steps: S1, extraction: uniformly mixing an animal-derived food sample with an extraction solvent, then adding a dewatering agent and buffer salt, uniformly mixing, and then carrying out solid-liquid separation to obtain an extracting solution; s2, purification: uniformly mixing the extracting solution in the step S1 with a purifying agent and a dewatering agent, and then carrying out solid-liquid separation to obtain a purified solution; s3, detecting: detecting and analyzing the purified liquid obtained in the step S2 by adopting a liquid chromatography-triple quadrupole tandem mass spectrometer; the animal-derived food is one or more of livestock meat, aquatic products, fat, liver, eggs, raw milk or casings. The application range of the method for detecting penthiopyrad and metabolite thereof is expanded to animal-derived food with different matrixes such as livestock meat