Capillary protein quantitative analysis method
The invention discloses a quantitative analysis method for capillary protein. The quantitative analysis method comprises the following steps: (1) filling a capillary with a dyeing-free glue reagent through a sampling device; the dyeing-free glue reagent comprises halide or trihalide; (2) adding a pr...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a quantitative analysis method for capillary protein. The quantitative analysis method comprises the following steps: (1) filling a capillary with a dyeing-free glue reagent through a sampling device; the dyeing-free glue reagent comprises halide or trihalide; (2) adding a protein sample at one end of the capillary tube; (3) after the dyeing-free glue reagent in the capillary tube is solidified, carrying out protein separation through electrophoresis; and (4) after protein separation, irradiating the protein fragments in the capillary by using fluorescence to carry out fluorescence detection so as to realize analysis and detection of the protein sample. According to the quantitative analysis method for the capillary protein, the glue-free reagent is added into the capillary for the first time, only a small amount of the glue-free reagent is needed to detect the protein sample, in addition, the glue-free reagent does not need to be dyed in the detection process, the detection time is fu |
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