Prokaryotic expression and purification method and application of seawater multi-hair recombinant protein with regeneration function

The invention discloses a prokaryotic expression and purification method of seawater multi-hair recombinant protein with a regeneration function. The method comprises the following steps: synthesizing a gene sequence by using a whole gene, and inserting a product containing a restriction enzyme site into a plasmid through PCR (Polymerase Chain Reaction) amplification, so as to construct a pET-30a-His-OxLumbricin prokaryotic expression vector; inducing for 16 hours under the conditions that the temperature is 15 DEG C and the IPTG is 0.5 mM; the method comprises the following steps: respectively detecting whole cells, supernatant and precipitate to finally obtain proteins with the molecular weight of 12kD which are massively distributed in lysis supernatant, indicating that the target protein is in soluble expression, and further purifying to obtain OxLumbricin protein with higher purity. The invention can provide a material for researching the action mechanism of OxLumb protein in regeneration of marine anima