CD56dimCD16 + NK cell culture kit, culture method and application

The invention provides a CD56dim CD16 + NK cell culture kit, a culture method and application. The kit comprises a stimulating factor A solution, an activation culture medium B solution and an amplification culture medium C solution, the stimulating factor A liquid comprises at least one of a CD16 antibody, an NKp30 antibody, an NKp44 antibody and an NKp46 antibody; the activated culture medium liquid B is prepared from IL-2 (Interleukin-2), IL-7 (Interleukin-7), IL-12 (Interleukin-12), IL-15 (Interleukin-15), IL-21 (Interleukin-21), L-ascorbic acid, 5-aminolevulinic acid, group A streptococcus OK432, a Euphoscopin C cell enhancer and autologous plasma; the amplification culture medium solution C is prepared from IL-2, IL-12, IL-15, L-ascorbic acid, an FMS-like tyrosine kinase 3 ligand, cyclodextrin and autologous plasma. Based on the culture kit and the culture method provided by the invention, after human peripheral blood mononuclear cells are co-cultured in vitro for 2-3 weeks, the proportion of CD56dim CD