Array chip for cell capture and tumor ball culture and preparation and operation method thereof

The invention discloses a cell capture and tumor ball culture array chip, which comprises a single cell sorting layer, a cell culture layer and a pipeline, the single cell sorting layer is bonded with the cell culture layer, the single cell sorting layer comprises at least one group of cell capture...

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Bibliographische Detailangaben
Hauptverfasser:	PANG LONG, GE YUXIN, YUAN HAOYUE, FAN JIANGLIN, FAN SHIGANG, GUO LULU, JIN YARU, CHEN DI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY ENZYMOLOGY METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDEDFOR SHAPING OR JOINING OF PLASTICS SPIRITS TRANSPORTING VINEGAR WINE WORKING OF PLASTICS WORKING OF SUBSTANCES IN A PLASTIC STATE, IN GENERAL
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Beschreibung
Zusammenfassung:	The invention discloses a cell capture and tumor ball culture array chip, which comprises a single cell sorting layer, a cell culture layer and a pipeline, the single cell sorting layer is bonded with the cell culture layer, the single cell sorting layer comprises at least one group of cell capture arrays, a cell suspension inlet and a cell suspension outlet, each group of cell capture arrays comprises at least three columns of microarrays, each column of microarrays comprises single cell capture units which are arranged in parallel, each single cell capture unit comprises two microstructures, a screening groove is formed between the two microstructures, and a first pore and a second pore which are used for capturing cells are respectively formed in two ends of the screening groove; the width of the first pores is 1-2 [mu] m greater than that of the second pores; a culture groove is formed in the cell culture layer and corresponds to the single cell capture unit. The invention also discloses a preparation met