Method for extracting tea saponin

The invention provides an extraction method of tea saponin, and belongs to the technical field of plant extraction. Comprising the following steps: (1) crushing camellia seed meal, adding into petroleum ether, heating, refluxing, degreasing, filtering, washing and drying to obtain degreased camellia...

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Hauptverfasser: WEN YING, ZHU BORONG, LIU MINGDENG, XU KEQIN, LIU YULIN, XU BING
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creator WEN YING
ZHU BORONG
LIU MINGDENG
XU KEQIN
LIU YULIN
XU BING
description The invention provides an extraction method of tea saponin, and belongs to the technical field of plant extraction. Comprising the following steps: (1) crushing camellia seed meal, adding into petroleum ether, heating, refluxing, degreasing, filtering, washing and drying to obtain degreased camellia seed meal powder; (2) adding the degreased camellia seed meal powder into water, adding a compound enzyme for enzymolysis, and performing enzyme deactivation and drying to obtain an enzymolysis product; and (3) adding the enzymatic hydrolysate into an ionic liquid-ethanol-water solution, carrying out ultrahigh pressure extraction, filtering, concentrating filtrate, dissolving with 70-80wt% ethanol, concentrating, adding acetone, filtering, drying, dissolving in 90-95wt% ethanol, and purifying with macroporous resin to obtain the tea saponin. The extraction method of the tea saponin is low in cost, convenient to experiment and relatively high in extraction rate and purity, and has a wide application prospect. 本发明提出
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Comprising the following steps: (1) crushing camellia seed meal, adding into petroleum ether, heating, refluxing, degreasing, filtering, washing and drying to obtain degreased camellia seed meal powder; (2) adding the degreased camellia seed meal powder into water, adding a compound enzyme for enzymolysis, and performing enzyme deactivation and drying to obtain an enzymolysis product; and (3) adding the enzymatic hydrolysate into an ionic liquid-ethanol-water solution, carrying out ultrahigh pressure extraction, filtering, concentrating filtrate, dissolving with 70-80wt% ethanol, concentrating, adding acetone, filtering, drying, dissolving in 90-95wt% ethanol, and purifying with macroporous resin to obtain the tea saponin. 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subjects CHEMISTRY
DERIVATIVES THEREOF
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
STEROIDS
SUGARS
title Method for extracting tea saponin
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