Method for extracting tea saponin
The invention provides an extraction method of tea saponin, and belongs to the technical field of plant extraction. Comprising the following steps: (1) crushing camellia seed meal, adding into petroleum ether, heating, refluxing, degreasing, filtering, washing and drying to obtain degreased camellia...
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creator | WEN YING ZHU BORONG LIU MINGDENG XU KEQIN LIU YULIN XU BING |
description | The invention provides an extraction method of tea saponin, and belongs to the technical field of plant extraction. Comprising the following steps: (1) crushing camellia seed meal, adding into petroleum ether, heating, refluxing, degreasing, filtering, washing and drying to obtain degreased camellia seed meal powder; (2) adding the degreased camellia seed meal powder into water, adding a compound enzyme for enzymolysis, and performing enzyme deactivation and drying to obtain an enzymolysis product; and (3) adding the enzymatic hydrolysate into an ionic liquid-ethanol-water solution, carrying out ultrahigh pressure extraction, filtering, concentrating filtrate, dissolving with 70-80wt% ethanol, concentrating, adding acetone, filtering, drying, dissolving in 90-95wt% ethanol, and purifying with macroporous resin to obtain the tea saponin. The extraction method of the tea saponin is low in cost, convenient to experiment and relatively high in extraction rate and purity, and has a wide application prospect.
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本发明提出</description><subject>CHEMISTRY</subject><subject>DERIVATIVES THEREOF</subject><subject>METALLURGY</subject><subject>NUCLEIC ACIDS</subject><subject>NUCLEOSIDES</subject><subject>NUCLEOTIDES</subject><subject>ORGANIC CHEMISTRY</subject><subject>STEROIDS</subject><subject>SUGARS</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZFD0TS3JyE9RSMsvUkitKClKTC7JzEtXKElNVChOLMjPy8zjYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxzn6GhuZGZiYGBpaOxsSoAQB9TyWX</recordid><startdate>20231222</startdate><enddate>20231222</enddate><creator>WEN YING</creator><creator>ZHU BORONG</creator><creator>LIU MINGDENG</creator><creator>XU KEQIN</creator><creator>LIU YULIN</creator><creator>XU BING</creator><scope>EVB</scope></search><sort><creationdate>20231222</creationdate><title>Method for extracting tea saponin</title><author>WEN YING ; ZHU BORONG ; LIU MINGDENG ; XU KEQIN ; LIU YULIN ; XU BING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117264009A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>CHEMISTRY</topic><topic>DERIVATIVES THEREOF</topic><topic>METALLURGY</topic><topic>NUCLEIC ACIDS</topic><topic>NUCLEOSIDES</topic><topic>NUCLEOTIDES</topic><topic>ORGANIC CHEMISTRY</topic><topic>STEROIDS</topic><topic>SUGARS</topic><toplevel>online_resources</toplevel><creatorcontrib>WEN YING</creatorcontrib><creatorcontrib>ZHU BORONG</creatorcontrib><creatorcontrib>LIU MINGDENG</creatorcontrib><creatorcontrib>XU KEQIN</creatorcontrib><creatorcontrib>LIU YULIN</creatorcontrib><creatorcontrib>XU BING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WEN YING</au><au>ZHU BORONG</au><au>LIU MINGDENG</au><au>XU KEQIN</au><au>LIU YULIN</au><au>XU BING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for extracting tea saponin</title><date>2023-12-22</date><risdate>2023</risdate><abstract>The invention provides an extraction method of tea saponin, and belongs to the technical field of plant extraction. Comprising the following steps: (1) crushing camellia seed meal, adding into petroleum ether, heating, refluxing, degreasing, filtering, washing and drying to obtain degreased camellia seed meal powder; (2) adding the degreased camellia seed meal powder into water, adding a compound enzyme for enzymolysis, and performing enzyme deactivation and drying to obtain an enzymolysis product; and (3) adding the enzymatic hydrolysate into an ionic liquid-ethanol-water solution, carrying out ultrahigh pressure extraction, filtering, concentrating filtrate, dissolving with 70-80wt% ethanol, concentrating, adding acetone, filtering, drying, dissolving in 90-95wt% ethanol, and purifying with macroporous resin to obtain the tea saponin. The extraction method of the tea saponin is low in cost, convenient to experiment and relatively high in extraction rate and purity, and has a wide application prospect.
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subjects | CHEMISTRY DERIVATIVES THEREOF METALLURGY NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY STEROIDS SUGARS |
title | Method for extracting tea saponin |
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