Method for detecting immunocompetence of T lymphocytes

The invention relates to the technical field of biology, in particular to a method for detecting immunocompetence of T lymphocytes. The invention provides a method for detecting the immunocompetence of T lymphocytes. The CD3 antibody and the CD28 antibody are used for stimulating T lymphocytes, and the CD4 antibody and the CD8 antibody are selected as a T lymphocyte preparation or a target spot for flow cytometry detection of a culture solution, so that the function and the activity of the T lymphocytes can be reflected. And the culture time is optimized, so that the content of IFN gamma in the T lymphocyte can be accurately detected, and reference is provided for evaluating the immunocompetence of the T lymphocyte. 本发明涉及生物技术领域，具体涉及检测T淋巴细胞免疫活性的方法。本发明提供了检测T淋巴细胞免疫活性的方法。本发明先通过CD3抗体和CD28抗体刺激T淋巴细胞，选择CD4和CD8抗体作为T淋巴细胞制剂或培养液流式细胞检测的靶点可以反应T淋巴细胞的功能与活性。且本发明对培养时间进行了优化，能够准确检测出T淋巴细胞中的IFNγ的含量，为评价T淋巴细胞的免疫活性提供参考。