Single-molecule mechanical testing system and method based on double-optical-trap optical tweezers

The invention provides a single-molecule mechanical testing system and method based on double-light-trap optical tweezers, and the system comprises a laser beam splitting module which is used for splitting a laser beam into two light beams with the polarization states being perpendicular to each other; the double-light-trap operation module is used for receiving the two light beams and independently operating deflection of each light beam so as to control a light trap formed by each light beam to move in the horizontal direction; the double-light-trap forming module is used for receiving the two light beams emitted from the double-light-trap operation module and generating two independent light traps through focusing of a microscope objective; the objective table can move in a three-dimensional manner and is used for placing the sample pool; the double-light-trap monitoring module is used for monitoring the number and the movement condition of particles in each light trap and the relative movement between the