Tandem co-expression double visual enhanced fluorescent protein reporter gene plant expression vector and application thereof

The invention provides a tandem co-expression double visual enhanced fluorescent protein reporter gene plant expression vector, which is characterized in that after an enhanced expression framework is obtained through gene synthesis on the basis of a starting vector, DsRed2 and eGFP are respectively inserted through homologous recombination reaction, and an enhanced DsRed2 expression cassette is inserted in the same direction on the basis of an enhanced eGFP expression cassette to obtain a final vector. A to-be-analyzed target gene sequence is cloned into the MCS site of the vector through homologous recombination reaction, and the obtained recombinant plasmid can be used for plant genetic transformation. The red fluorescence signal and the green fluorescence signal are clear and stable in imaging effect through observation, and are mutually independent and mutually characterized. The homonymous tandem co-expression dual-visual enhanced fluorescent protein reporter gene plant expression vector can improve the