Application of camellia oleifera PAP gene in improvement of phosphorus absorptivity of plants
The invention belongs to the technical field of gene engineering, and particularly relates to application of a camellia oleifera PAP gene in improvement of phosphorus absorptivity of plants, and the PAP gene is any one of CoPAP1 and CoPAP2; the nucleotide sequence of the CoPAP1 gene is as shown in S...
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creator | LIU WENXIN LIU YANAN XIAO TAIPING YE SICHENG |
description | The invention belongs to the technical field of gene engineering, and particularly relates to application of a camellia oleifera PAP gene in improvement of phosphorus absorptivity of plants, and the PAP gene is any one of CoPAP1 and CoPAP2; the nucleotide sequence of the CoPAP1 gene is as shown in SEQ ID1; the nucleotide sequence of the CoPAP2 gene is as shown in SEQ ID 2. CoPAP1 and CoPAP2 genes are cloned and are subjected to construction of a fusion GFP expression vector, real-time fluorescence quantification (qRT-PCR) is utilized to reveal an expression mode of responding to low phosphorus stress, and the phosphorus absorption capacity of arabidopsis thaliana is improved by transforming arabidopsis thaliana.
本发明属于基因工程技术领域,具体为油茶PAP基因在提高植物磷吸收性中的应用,所述PAP基因为CoPAP1、CoPAP2中的任一个;所述CoPAP1基因的核苷酸序列如SEQ ID1所示;所述CoPAP2基因的核苷酸序列如SEQ ID2所示。通过克隆CoPAP1、CoPAP2基因,并对其进行融合GFP表达载体的构建,利用实时荧光定量(qRT-PCR)揭示其响应低磷胁迫的表达模式,通过转化拟南芥提高了拟南芥的磷吸收能力。 |
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本发明属于基因工程技术领域,具体为油茶PAP基因在提高植物磷吸收性中的应用,所述PAP基因为CoPAP1、CoPAP2中的任一个;所述CoPAP1基因的核苷酸序列如SEQ ID1所示;所述CoPAP2基因的核苷酸序列如SEQ ID2所示。通过克隆CoPAP1、CoPAP2基因,并对其进行融合GFP表达载体的构建,利用实时荧光定量(qRT-PCR)揭示其响应低磷胁迫的表达模式,通过转化拟南芥提高了拟南芥的磷吸收能力。</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231208&DB=EPODOC&CC=CN&NR=117187277A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231208&DB=EPODOC&CC=CN&NR=117187277A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU WENXIN</creatorcontrib><creatorcontrib>LIU YANAN</creatorcontrib><creatorcontrib>XIAO TAIPING</creatorcontrib><creatorcontrib>YE SICHENG</creatorcontrib><title>Application of camellia oleifera PAP gene in improvement of phosphorus absorptivity of plants</title><description>The invention belongs to the technical field of gene engineering, and particularly relates to application of a camellia oleifera PAP gene in improvement of phosphorus absorptivity of plants, and the PAP gene is any one of CoPAP1 and CoPAP2; the nucleotide sequence of the CoPAP1 gene is as shown in SEQ ID1; the nucleotide sequence of the CoPAP2 gene is as shown in SEQ ID 2. CoPAP1 and CoPAP2 genes are cloned and are subjected to construction of a fusion GFP expression vector, real-time fluorescence quantification (qRT-PCR) is utilized to reveal an expression mode of responding to low phosphorus stress, and the phosphorus absorption capacity of arabidopsis thaliana is improved by transforming arabidopsis thaliana.
本发明属于基因工程技术领域,具体为油茶PAP基因在提高植物磷吸收性中的应用,所述PAP基因为CoPAP1、CoPAP2中的任一个;所述CoPAP1基因的核苷酸序列如SEQ ID1所示;所述CoPAP2基因的核苷酸序列如SEQ ID2所示。通过克隆CoPAP1、CoPAP2基因,并对其进行融合GFP表达载体的构建,利用实时荧光定量(qRT-PCR)揭示其响应低磷胁迫的表达模式,通过转化拟南芥提高了拟南芥的磷吸收能力。</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjDEKAjEQRbexEPUO4wEsokVsw6JYyRa2soxhogPZzJDEBW_vKh7A4vOK9_jz5upUI3usLAkkgMeBYmQEicSBMkLnOrhTIuAEPGiWkQZK9RPrQ8q0_CyAtyJZK49cX18VMdWybGYBY6HVj4tmfTxc2tOGVHoqin46rn17Nsaavd1a63b_NG_21zxy</recordid><startdate>20231208</startdate><enddate>20231208</enddate><creator>LIU WENXIN</creator><creator>LIU YANAN</creator><creator>XIAO TAIPING</creator><creator>YE SICHENG</creator><scope>EVB</scope></search><sort><creationdate>20231208</creationdate><title>Application of camellia oleifera PAP gene in improvement of phosphorus absorptivity of plants</title><author>LIU WENXIN ; LIU YANAN ; XIAO TAIPING ; YE SICHENG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117187277A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIU WENXIN</creatorcontrib><creatorcontrib>LIU YANAN</creatorcontrib><creatorcontrib>XIAO TAIPING</creatorcontrib><creatorcontrib>YE SICHENG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIU WENXIN</au><au>LIU YANAN</au><au>XIAO TAIPING</au><au>YE SICHENG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Application of camellia oleifera PAP gene in improvement of phosphorus absorptivity of plants</title><date>2023-12-08</date><risdate>2023</risdate><abstract>The invention belongs to the technical field of gene engineering, and particularly relates to application of a camellia oleifera PAP gene in improvement of phosphorus absorptivity of plants, and the PAP gene is any one of CoPAP1 and CoPAP2; the nucleotide sequence of the CoPAP1 gene is as shown in SEQ ID1; the nucleotide sequence of the CoPAP2 gene is as shown in SEQ ID 2. CoPAP1 and CoPAP2 genes are cloned and are subjected to construction of a fusion GFP expression vector, real-time fluorescence quantification (qRT-PCR) is utilized to reveal an expression mode of responding to low phosphorus stress, and the phosphorus absorption capacity of arabidopsis thaliana is improved by transforming arabidopsis thaliana.
本发明属于基因工程技术领域,具体为油茶PAP基因在提高植物磷吸收性中的应用,所述PAP基因为CoPAP1、CoPAP2中的任一个;所述CoPAP1基因的核苷酸序列如SEQ ID1所示;所述CoPAP2基因的核苷酸序列如SEQ ID2所示。通过克隆CoPAP1、CoPAP2基因,并对其进行融合GFP表达载体的构建,利用实时荧光定量(qRT-PCR)揭示其响应低磷胁迫的表达模式,通过转化拟南芥提高了拟南芥的磷吸收能力。</abstract><oa>free_for_read</oa></addata></record> |
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subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
title | Application of camellia oleifera PAP gene in improvement of phosphorus absorptivity of plants |
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