Application of camellia oleifera PAP gene in improvement of phosphorus absorptivity of plants

The invention belongs to the technical field of gene engineering, and particularly relates to application of a camellia oleifera PAP gene in improvement of phosphorus absorptivity of plants, and the PAP gene is any one of CoPAP1 and CoPAP2; the nucleotide sequence of the CoPAP1 gene is as shown in S...

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Bibliographische Detailangaben
Hauptverfasser: LIU WENXIN, LIU YANAN, XIAO TAIPING, YE SICHENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of gene engineering, and particularly relates to application of a camellia oleifera PAP gene in improvement of phosphorus absorptivity of plants, and the PAP gene is any one of CoPAP1 and CoPAP2; the nucleotide sequence of the CoPAP1 gene is as shown in SEQ ID1; the nucleotide sequence of the CoPAP2 gene is as shown in SEQ ID 2. CoPAP1 and CoPAP2 genes are cloned and are subjected to construction of a fusion GFP expression vector, real-time fluorescence quantification (qRT-PCR) is utilized to reveal an expression mode of responding to low phosphorus stress, and the phosphorus absorption capacity of arabidopsis thaliana is improved by transforming arabidopsis thaliana. 本发明属于基因工程技术领域,具体为油茶PAP基因在提高植物磷吸收性中的应用,所述PAP基因为CoPAP1、CoPAP2中的任一个;所述CoPAP1基因的核苷酸序列如SEQ ID1所示;所述CoPAP2基因的核苷酸序列如SEQ ID2所示。通过克隆CoPAP1、CoPAP2基因,并对其进行融合GFP表达载体的构建,利用实时荧光定量(qRT-PCR)揭示其响应低磷胁迫的表达模式,通过转化拟南芥提高了拟南芥的磷吸收能力。